Research Fellow Vacancy

>> Background

A talented and creative researcher in the field of evolutionary developmental biology is sought to take part in a highly interdisciplinary and international project funded by the Human Frontier Science Program (HFSP). The overall aim of the project is to understand the role of gene family expansions in the evolution of novel complex traits in molluscs. We are particularly interested in studying a class of receptors called pattern recognition receptors (PRR) that includes gene families such as Toll-like receptors and C-type lectin receptors. PRRs are best known as central components of innate immunity, detecting conserved molecular patterns and directing downstream processes such as phagocytosis, inflammation, and antimicrobial responses. Preliminary data from two independently studied molluscan systems—biomineralisation in snails (Sleight) and kleptocnidy in nudibranchs (Goodheart)—suggest that PRR gene expansion may drive evolutionary novelty outside of a classic innate immune context.

>> Project

Broadly, the project will aim to understand the role of gene family expansions in the evolution of novel complex traits in molluscs – with a focus on pattern recognition receptors and biomineralization.

>>Approaches

Our approach is to use a combination of state-of-the-art techniques, such as CRISPR/Cas9 gene knock-out and single cell technologies, to understand the functional role of specific genes in mollusc shell morphogenesis and development. This project will involve:

(i) analysing single cell RNAseq atlases to determine the cell type expression specificity of PRR genes

(ii) determining the spatial expression localisation of PRR genes in developmental stages of molluscs using HCR in situ hybridisations (HCR-ISH) 

(iii) CRISPR-Cas9 gene knock-out functional analysis of specific significantly expanded PRR genes 

>> Training and opportunities

You will be based in the Sleight Lab at the University of Aberdeen, a supportive environment where curiosity is encouraged and nurtured. The Sleight Lab is a friendly space that values collaboration and is dedicated to the scientific success of all its members. 

The successful candidate will join a research team, led by Dr Vicky Sleight, who uses experimental and bioinformatic approaches to understand fundamental questions in developmental biology and evolution. The primary model system studied in the Sleight lab is Crepidula fornicata and this species will form the basis of the in vivo functional analyses in the project. The project is part of an interdisciplinary collaboration with Dr Jessica Goodheart’s team at the American Museum of Natural History in the USA, who will identify and reconstruct PRR gene families across molluscs, and Dr Manabu Bessho‑Uehara’s team at Tohoku University in Japan, who will computationally and biochemically characterize molluscan PRRs and identify their ligands. There is considerable flexibility available in project direction and the successful candidate will evidence independence in project design and delivery. Successful candidates will have the opportunity and mentorship available to develop their own ideas and shape the project direction from an early stage.

The Sleight lab is based at the University of Aberdeen, located in an affordable and vibrant city with access to some of the best outdoor activities and quality of life that the UK has to offer.

>> Candidate background

We are seeking a talented individual who is motivated to understand how embryos develop and how developmental processes evolve. Since the project will involve advanced experimental embryology and bioinformatic techniques, it is essential to have at least some experience in both experimental work with embryos and bioinformatic data analysis (preferably of single cell data). An ability to work with small delicate embryos is essential, although prior experience specifically with molluscs, or our laboratory species, Crepidula fornicata, is not required. An ideal candidate would have a PhD, or postdoctoral experience, in developmental biology or evolutionary developmental biology (this includes PhD students who are near completion and will have sustained a PhD degree by the start date). However, talented candidates from a wide range of disciplines are encouraged to apply – provided they can evidence enthusiasm and commitment to learning about evolutionary developmental biology.

More generally, you must show an aptitude and commitment to working in a team; the collaborative nature of this project makes communication skills highly valuable. It is essential that the candidate has a proven track record of high-quality research in their subject area, for example evidenced by publications from their PhD work. We are looking to train the next generation of scientific leaders, who will contribute to society in fields both within and outside academia, and so are looking for a gifted candidate who has a high degree of creativity, independence and initiative. Over the course of the project, you will receive substantial mentorship and guidance that will help you to develop your own interests and career plans for your next step.